FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357571
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT39650.16818157332271225TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA7450.051.262511
ATCGGAA7950.047.2566342
TCGGAAG8750.042.8904843
CGGAAGA9450.039.7083554
GGTCGCG500.001616288137.999394
TATGCCG5450.032.68018748-49
CGTATGC5700.031.66347346-47
CTCGTAT5750.030.97447844-45
TCTCGTA5500.030.65599842-43
TCGTATG6550.027.9164444-45
TGCCGTC6550.027.19191450-51
ACGACGC1056.853937E-527.1747022
CGACGCT1257.1133545E-626.7333981
ATGCCGT6700.026.58313848-49
ACTCCGG7600.024.999632-33
CCGGAGA7600.024.99853934-35
GCCGTCT6800.024.7952950-51
ATCTCGT7000.024.42610742-43
CACTCCG7700.024.05805430-31
CGTCTGA7750.023.89979716-17
Produced by FastQC (version 0.11.5)