FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4064580
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2700.035.40880258-59
GTCGCCG3650.026.83149756-57
TCGCCGT3600.026.55643856-57
GAGCGTC7750.025.8743349
GCGTCGT4150.023.59776910-11
AGAGCGT8750.023.4496528
GCCGTAT4400.021.72812858-59
GGTCGCC4650.021.06231954-55
CGCTAAT2551.232911E-820.4333741
AGCGTCG4750.020.12611810-11
CGTCGTG4650.020.05774712-13
TGGTCGC4800.019.91830854-55
CCGTATC4950.019.7848460-61
GTGGTCG5100.019.66256552-53
GATCGGA10250.019.409441
ATCGGAA10150.019.2996672
CGTATCA5350.018.305660-61
TCGGAAG11200.017.9036753
AAGAGCG11850.017.7088857
CGGAAGA11800.017.3885124
Produced by FastQC (version 0.11.5)