FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4459597
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4950.023.98716244-45
TCTCGTA5250.021.71253842-43
TCGTATG5700.021.66419244-45
CGTATGC5750.021.47629246-47
TATGCCG5450.021.35149248-49
ATGCCGT6550.018.49087748-49
GTCACGA6950.017.42841328-29
TGCCGTC6900.016.86430250-51
GATCGGA15600.015.595271
TCGGAAG15250.015.5753123
ACGAGAT7700.015.42256532-33
ATCGGAA15550.015.2928312
ATCTCGT7400.015.08324542-43
GCCGTCT7750.015.01466850-51
AGTCACG8550.013.88917228-29
CGGAAGA17100.013.8883934
TCCGATC2500.001452820413.3568911
CATCTCG9200.012.906569540-41
CGTCTTC9650.012.55052152-53
CGTCTGA9300.012.51138116-17
Produced by FastQC (version 0.11.5)