FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5915688
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA853.6498022E-739.1098678
TATGCCG5700.024.16308448-49
CGGCTAT6850.020.456234-35
TCGCCGT1656.338084E-520.1475077
CTCGTAT7050.019.53611244-45
GTCGCCG1951.0356098E-519.4833036
CGTATGC7450.018.16829546-47
TGCCGTC7800.017.35290350-51
GATCGGA16050.016.9583971
TCGTATG7600.016.24759144-45
TCACCGG8550.015.83419930-31
ATCGGAA17650.015.6280182
ACCGGCT8750.015.47207832-33
TCGGAAG18200.015.1390873
CGTTGGA24150.015.0273171
ACGGGAT16450.015.0122119
TCTCGTA7800.014.91803842-43
GGTCGCC2601.2200895E-414.6124785
CGGAAGA19000.014.2493324
CCGAATC4352.298475E-814.2126392
Produced by FastQC (version 0.11.5)