FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3406525
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4750.033.4972948-49
CTCGTAT5500.030.22549244-45
TCGGAAG11650.027.7216553
GATCGGA11550.027.6128851
ATCGGAA11750.027.1269152
TCGTATG5800.027.02427144-45
CGTATGC6150.025.8724646-47
CGGAAGA13150.025.2825684
TCTCGTA6000.024.93584842-43
ATGCCGT6600.023.3882648-49
ATCTCGT6900.022.71588742-43
TGCCGTC6750.022.51735750-51
GCCGTCT6750.021.11002350-51
CCGTCTT7700.018.81410452-53
ACACGTC9400.017.4315912-13
CGTCTTC7500.017.09924552-53
CGTCTGA9650.016.73353816-17
GTATGCC10350.015.14403746-47
ACGTCTG10850.015.1016914-15
AATCTCG10200.014.66760840-41
Produced by FastQC (version 0.11.5)