FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614633
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC1156.730261E-1140.5550962
CGCCGTA3150.039.9707758-59
GAGCGTC6500.038.7412729
GTCGCCG3550.034.8095656-57
GATCGGA7750.033.6210371
AGAGCGT7700.033.309298
TCGCCGT3750.032.9530556-57
TCGGAAG8150.032.0417063
ATCGGAA8300.031.4668462
CACGACG1701.10958354E-1030.654481
CGGAAGA8200.030.1402744
GCCGTAT4150.029.21558458-59
TGGTCGC4400.028.61543754-55
CCGTGTA4750.027.96071638-39
GGTCGCC4450.027.76995754-55
AAGAGCG9150.027.5211227
GTGGTCG4500.026.42587952-53
GCGTCGT4700.026.29308110-11
AGCGTCG4850.025.96064810-11
GAGCCGT5650.025.3977736-37
Produced by FastQC (version 0.11.5)