FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3530110
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT59420.1683233666939557TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA12900.053.63681
ATCGGAA13950.049.08782
TCGGAAG14750.046.375433
ACGACGC1201.8189894E-1243.590992
CGGAAGA15500.043.5135884
TATGCCG9450.035.18063748-49
TCTCGTA9300.033.70627642-43
CTCGTAT9650.033.46717544-45
ATGCCGT10150.033.45626448-49
CGTATGC10000.033.00776746-47
TTACTCG10600.032.93579534-35
TGCCGTC10350.032.58078850-51
TACTCGA10350.032.35111636-37
TCGTATG10600.032.2599844-45
CGACGCT1950.031.812171
GCCGTCT11000.031.7349850-51
CACGACG1701.03682396E-1030.8765161
ACACGTC12550.028.19316512-13
ACTCGAT11550.027.96195236-37
ATCTCGT10900.027.88709342-43
Produced by FastQC (version 0.11.5)