FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3530110
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT45770.12965601638475854Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC12350.052.1081439
GATCGGA13400.049.1408271
AGAGCGT13500.047.6686258
ATCGGAA14500.045.3504372
TCGGAAG14600.044.7140543
AAGAGCG15900.041.0599337
ACGACGC2300.040.553892
CGCCGTA7700.039.97143658-59
CGGAAGA16350.039.9281464
GTCGCCG8300.037.64324656-57
TCGCCGT8450.037.52689456-57
CGACGCT2550.036.5728383
CACGACG2500.036.0036131
GGTCGCC9300.034.84964854-55
TGGTCGC8900.034.84401354-55
GCGTCGT9300.034.3479710-11
AGCGTCG9500.033.87029310-11
GTGGTCG9600.033.0338252-53
CGTCGTG9700.032.6907212-13
GCCGTAT9800.032.35782658-59
Produced by FastQC (version 0.11.5)