FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3063052
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT30690.10019418540723436TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC1700.058.740982
CACGACG1750.057.288331
CGACGCT2700.040.4677623
GATCGGA10150.035.7464181
ATCGGAA10500.034.871632
TCGGAAG11300.032.3710983
CGGAAGA11350.031.8047474
TATGCCG5550.031.66111848-49
CTCGTAT5950.030.3315644-45
TTACTCG6350.030.29342834-35
TCGTATG6500.029.9570244-45
TCTCGTA5750.028.90986642-43
CGTATGC6350.028.42021646-47
GCCGTCT7250.025.54724750-51
TACTCGA7000.025.44134536-37
ATGCCGT7350.024.8765948-49
ATCTCGT6750.024.27510642-43
ACTCGAT7300.024.07053236-37
GACGCTC4200.023.748944
TGCCGTC7550.023.58858150-51
Produced by FastQC (version 0.11.5)