FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5418792
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2350.029.30300958-59
GTCGCCG3350.022.68178456-57
TCGCCGT3600.020.44707556-57
CGACGCT1456.1555643E-419.652576
GCCGTAT4250.017.87899658-59
CACGACG1600.001192849417.810144
ACGACGC1600.001192849417.810145
GGTCGCC4900.016.47543354-55
TGGTCGC4750.015.99596354-55
CCGTATC5100.013.96796660-61
GTGGTCG6250.012.53683652-53
CGTATCA6550.012.3259360-61
GTCTTCG7000.011.19587630-31
TCTTCGC6750.010.90757432-33
GAGCGTC13000.010.5957299
CGTTGGA20050.010.3476531
AGAGCGT15150.09.7189038
TTCGCCT7500.09.49988234-35
TCTCGGT8550.09.44358648-49
TCGGTGG8800.09.17487950-51
Produced by FastQC (version 0.11.5)