FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3912830
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT58960.15068377619267895TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA11900.054.1429371
ATCGGAA12600.051.3226362
TCGGAAG13050.049.1382373
CGGAAGA14350.044.683264
TATGCCG8550.036.1053448-49
CTCGTAT9100.033.6630644-45
ATGCCGT9600.032.89838448-49
TATCTCG9250.032.09151540-41
CGTATGC9700.032.06920246-47
TGCCGTC9650.031.98929250-51
TCTCGTA9450.031.41192642-43
TCGTATG9950.031.0259844-45
GTCACCG10600.030.02492128-29
CGCTCAT10250.029.65871834-35
ACCGCTC10800.029.2501132-33
GCCGTCT10750.028.7159750-51
CGTCTGA11450.028.41121716-17
CACCGCT11400.027.918830-31
ATCTCGT10650.027.42659242-43
CCGCTCA12500.024.89206332-33
Produced by FastQC (version 0.11.5)