FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4332093
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT1551.3542194E-624.6343251
TATGCCG4450.024.01342448-49
CGTATGC4850.022.52229546-47
CTCGTAT5400.020.66810444-45
TCTCGTA5100.020.02234342-43
TCGTATG6050.018.05506144-45
CGGCTAT6550.017.04171234-35
GCCGTCT6750.016.53467450-51
ACCGGCT7700.015.42245632-33
TGCCGTC6800.015.36545250-51
ATGCCGT6950.014.69214548-49
TCACCGG7600.014.68811430-31
CGTTGGA18300.014.60559751
ATCTCGT7500.014.5650942-43
ACGGGAT13300.013.5687069
AACGGGA17450.013.3354348
AAACGGG21400.013.0931687
GTCACCG9000.012.666903528-29
CGGAAGA20050.012.5554754
CCGTCTT9150.012.45737952-53
Produced by FastQC (version 0.11.5)