FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2994696
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT37010.12358516523880889Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGA652.5925328E-643.8418354
CGCCGTA4850.042.5953458-59
TCGGACG951.9323124E-839.9947243
GTCGCCG5700.038.32475756-57
TCGCCGT5550.037.221456-57
GCCGTAT6050.034.5391758-59
TGGTCGC6700.032.95794354-55
GGTCGCC6700.032.60355854-55
ATCGGAC1351.2001692E-831.6624892
GTGGTCG6850.031.19713852-53
CCGTATC6900.030.97236660-61
CGTATCA6950.030.74954460-61
GAGCGTC14100.026.949059
TCGGTGG8400.026.85837250-51
TCTCGGT8450.025.85630648-49
AGAGCGT15650.024.5834758
CTCGGTG9300.023.49309748-49
AAGAGCG16900.023.3261077
CGGTGGT9750.023.1395250-51
GATCGGA19350.022.1930831
Produced by FastQC (version 0.11.5)