FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5265882
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20850.015.3446541
ACGGGAT13650.014.6124579
TATGCCG4200.014.13447648-49
ATCGGAA8650.013.1959722
GATCGGA8450.012.9974911
CTCGTAT4550.012.00354644-45
AACGGGA19350.011.2897278
CGTATGC5100.011.17444346-47
TCGGAAG10350.011.0161573
TCTCGTA4351.8189894E-1110.91807942-43
AAACGGG22600.010.5067457
CGGAAGA11800.010.4654964
TGCCGTC6400.010.0178150-51
GAAACGG26300.09.2091826
GGAAACG26750.09.0542615
CCCCGAA6453.7534526E-58.8840281
ATGCCGT5359.586074E-108.87697948-49
ATCTCGT6801.8189894E-128.73044942-43
CCCAAAG118450.08.5062041
CCGAGTA25950.08.4307592
Produced by FastQC (version 0.11.5)