FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5265882
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2050.025.02427158-59
TCGCCGT2800.017.48813856-57
GTCGCCG3450.016.89675356-57
GAGCGTC7800.013.7513579
CGTTGGA21350.013.5362811
GCGTCGT3950.012.98697210-11
GATCGGA8350.012.4825581
CGTCGTG4350.012.3285112-13
AGCGTCG4550.012.29935210-11
AACGGGT5706.159098E-912.2721448
GCCGTAT4200.012.21422858-59
CGTAGAT3309.325744E-411.4853291
TGGTCGC5650.011.14325554-55
ATCGGAA9700.011.0590522
TCGGAAG10150.011.0265863
CCCGAAT8601.6370905E-1111.0179021
GGTCGCC4950.010.83476354-55
GAAACGG24300.010.5550556
AAACGGG23100.010.4977317
ACGGGTA3600.002198931810.3633419
Produced by FastQC (version 0.11.5)