FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3591294
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4100.027.22096346-47
ACGACGC908.882536E-426.4237482
TATGCCG4050.025.21209548-49
CTCGTAT4450.024.01258744-45
CGACGCT1000.001652486423.7545453
ATGCCGT4800.023.2515648-49
TCGTATG5450.022.65652544-45
GATCGGA9850.021.3322681
TCTCGTA5650.019.33298342-43
ATCTCGT5700.019.16339342-43
ATCGGAA10950.019.111972
TCGGAAG11450.018.2567673
TGCCGTC5800.018.01441650-51
CGGAAGA11850.017.6370664
CGTTGGA15450.016.382041
GCCGTCT6900.016.17550-51
AACGGGA13950.016.0004048
ACGGGAT11100.015.8301899
AAACGGG17250.014.86661157
CGTCTTC7900.014.12782752-53
Produced by FastQC (version 0.11.5)