FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3591294
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2700.033.68344558-59
GTCGCCG3450.028.38832956-57
TCGCCGT3500.027.98277956-57
GAGCGTC7850.024.3586859
GCCGTAT4400.023.31930758-59
GGTCGCC4650.022.56691654-55
TGGTCGC4500.022.2827454-55
GCGTCGT5100.021.03280810-11
GTGGTCG5000.020.988452-53
AGAGCGT9000.020.7274078
GATCGGA11300.019.286681
TCGGAAG11250.018.6544063
ATCGGAA12000.017.8778882
CGTATCA5750.017.84434160-61
CGTCGTG5400.017.70453612-13
AAGAGCG11350.017.6685187
AGCGTCG5300.017.59923410-11
CGGAAGA11300.017.3337424
CCGTATC5500.016.95949760-61
CGTTGGA15150.016.887261
Produced by FastQC (version 0.11.5)