FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5361876
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4100.020.8522348-49
GATCGGA10400.018.7636151
ATCGGAA10900.017.8923652
CTCGTAT5250.017.64222344-45
CGTATGC5100.016.76394846-47
TCTCGTA5300.016.13127142-43
TCGGAAG12150.016.0267373
TCGTATG5800.015.15031944-45
GTCACGA6400.013.73157728-29
CGGAAGA15900.013.4416614
CGTTGGA17700.013.1761571
ATGCCGT6650.012.49914548-49
AGTCACG7900.012.02628828-29
AACGGGA17100.011.6684258
TGCCGTC7250.010.80990750-51
ACGAGAT8450.010.39816332-33
ATCTCGT8150.010.19887842-43
GAAACGG22100.09.8883696
AAACGGG22150.09.8660477
CGAGATT11150.09.58405432-33
Produced by FastQC (version 0.11.5)