FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5361876
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2150.037.54962558-59
GTCGCCG3550.024.7481456-57
ACGACGC1801.9573781E-723.7455025
TCGCCGT3600.023.74483956-57
GCCGTAT3900.021.3092758-59
TGGTCGC4600.021.16328454-55
CACGACG2108.6354703E-720.3532894
GATCGGA9600.018.596071
CGACGCT1801.2361843E-418.4687236
GAGCGTC9250.017.9717489
GTGGTCG5450.016.9916452-53
GGTCGCC5350.016.8650254-55
ATCGGAA11250.016.0421542
AGAGCGT10750.015.9048548
CGTTGGA19900.015.7598081
GCGTCGT5400.015.39368910-11
CGTCGTG5900.014.49229412-13
ACGGGAT14400.014.1830629
AACGGGA19450.014.1626348
AGCGTCG6100.014.0165510-11
Produced by FastQC (version 0.11.5)