FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623519
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2800.040.70747458-59
TCGCCGT3300.033.81816556-57
TGGTCGC3500.032.56194754-55
GCCGTAT3450.032.3496658-59
GTCGCCG3550.032.10546556-57
GAGCGTC7700.030.2271189
GGTCGCC3900.029.2222654-55
CCGTATC4000.028.49550460-61
GTGGTCG4150.028.03373552-53
AGAGCGT8400.027.7060788
CGTATCA4200.027.13857760-61
ATCGGAA9050.026.2374532
GATCGGA9200.025.6966231
CGGAAGA9950.023.8651264
TCGGAAG10050.023.627663
AAGAGCG10200.023.2806427
GCGTCGT5750.020.65324810-11
GTCGTGT5650.020.5988112-13
CGTCGTG5650.020.5988112-13
TCGTGTA6450.018.78058214-15
Produced by FastQC (version 0.11.5)