FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048941
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA7700.046.993771
GAGCGTC7300.046.8452229
ATCGGAA8100.043.385582
CGCCGTA3950.041.47759258-59
TCGCCGT4000.039.771956-57
TCGGAAG8950.039.265163
AGAGCGT9150.036.8540958
CGTCGTG4850.034.76728412-13
GTCGCCG4600.034.06807756-57
GCGTCGT4900.033.92616310-11
GTACGTC5050.033.85870430-31
AGCGTCG5200.032.88228210-11
CGGAAGA10550.032.8606534
GTGTACG5650.031.93913328-29
AAGAGCG10900.031.8054967
GCCGTAT5450.031.80445158-59
TGGTCGC5200.030.5935254-55
GGTCGCC5750.028.90601554-55
TACGTCC5900.028.17781432-33
CGTATCA6400.027.4551660-61
Produced by FastQC (version 0.11.5)