FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6458729
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6150.026.25790248-49
CTCGTAT7750.021.1437544-45
TCGTATG8150.019.23184644-45
TCTCGTA8450.018.82988542-43
CGTATGC8650.018.11989846-47
GTCACGA10100.016.93185628-29
ATGCCGT9400.016.67409748-49
TGCCGTC9400.016.42158750-51
GCCGTCT10100.016.22397650-51
CGTTGGA22750.015.4807991
ATCTCGT10750.015.022078542-43
ACGAGAT11900.014.36828832-33
GATCGGA24450.014.209771
AGTCACG11850.014.03049928-29
CGGAAGA25550.013.94210154
TCGGAAG25850.013.596143
ATCGGAA26250.013.5904082
AAACGGG27050.012.8211757
AACGGGA22800.012.7106358
CGTCTTC13350.012.27436652-53
Produced by FastQC (version 0.11.5)