FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4960610
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA19000.013.5256331
ACGGGAT13850.013.0348559
AAACGGG22300.011.0782427
AACGGGA18600.010.9831748
GAAACGG23550.010.288496
GGAAACG25650.09.8158045
GTTGGAA48050.06.63266942
AAAGCGC11601.245486E-46.1509222
CCCAAAG106850.06.05733781
CGGGATT14900.05.73920910-11
GACCTCG14951.9831848E-55.71842864
GATCGGA8555.0352664E-65.55521992-93
CAAAGCG13802.1838372E-45.5177031
AAGCGCT13802.2483603E-45.5064693
CCCAAGT57050.05.50561331
CCGGGTT20951.9364415E-75.44921732
CAAGCGA20255.7800025E-75.3960419
TCGGAAG10156.1051287E-75.381501794-95
ATCGGAA8951.1212775E-55.30699594-95
CCAAAGT101400.05.2539632
Produced by FastQC (version 0.11.5)