FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13111684
Sequences flagged as poor quality0
Sequence length35-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT318480.2428978611748117No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT152060.1159728986757155No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC150210.11456194337813509No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGTCT189350.027.1401183
TGGAGTC194200.026.5363332
GAGTCTT197000.026.19534
GTCTTGG201350.025.9139446
AGTCTTG216850.024.0619435
TCTTGGA239900.022.4673677
TCGCTAT23300.021.2548892
CTTGGAA249850.020.8832238
CTCGCTA23100.020.8217031
CCCGATT16950.020.7529491
TTGGAAG252350.020.6194559
CCCGTAT24350.020.3424641
CGGGTAT24100.019.9577331
GTCTGGA150000.019.478551
CGCTATG24600.018.3810123
GTCGGGT16800.017.9470651
GCCCGTT19900.017.6765081
GCCGTAT13100.017.5360641
TACGTTC105900.017.33785625-29
CCCGTCT73650.016.8626881
Produced by FastQC (version 0.11.5)