FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13111684
Sequences flagged as poor quality0
Sequence length41-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG72350.036.942243
GGCGCGT78100.033.935554
GGGCGAT24950.032.8730741
CGCGTGC83400.031.854166
GTGGCGC90200.030.5247062
GCGCGTG88400.030.2970475
GCGTGCC91600.029.0013717
GGCGATC27850.028.652142
CGTGCCT108550.024.8014628
CGGTGGC89800.021.8721921
CGATCTG38200.021.622914
TGCGACA38050.020.7571896
GCGATCT40250.020.5281963
GCGACAT42000.020.0008687
CGACATC47900.017.0861878
GCTGCGA47150.016.2997784
CTGCGAC49200.015.7616765
CAACCCG30550.014.3347849
ACGATAC13850.013.47935
CGGAACG13150.013.130811
Produced by FastQC (version 0.11.5)