FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258561
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGTT2000.004454224430.897472145
CGTCGAG2357.999006E-826.0439531
TATAGTA2902.3515895E-823.4495355
GACTTGT11400.023.2644083
TGATCAA12250.022.2052759
GAGACTT12550.021.6744731
CTACTCG6150.021.009262
CCCGAAT1954.031127E-420.92421
AGACTTG14000.020.4010982
GCCGGTT2004.7820297E-420.4010969
AAAGTGC19450.019.9290924
ACTTGTG13800.019.7112054
GTCGAGA3201.5656078E-619.1260261
CTTGTGA14600.019.0969165
CGAGTAG4104.5163688E-818.2448833
CGAGACT10850.018.1760921
TACTCGG7500.018.1343083
ACGTCGA2250.001056271618.1343081
AAGTGCT20400.018.0009695
GTGATCA15250.017.8370258
Produced by FastQC (version 0.11.5)