FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258561
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC19200.021.2556424
CCAAAGT20800.020.6016242
AAGTGCT20300.019.7687955
GCTACTT10000.019.0450551
CAAAGTG24700.018.1748663
CTACTCG5301.4188117E-1017.9670332
CCCAAAG26300.017.5864181
GCTACTC12650.016.6684551
GTATACT3706.24343E-616.5449331
CTACTTG8450.016.0989482
ATGATAG4351.6705999E-615.6363344
ATACTCG2650.003150469215.4003134
GCTGGGA43900.015.3389249
TTGGGAG24250.014.8658036
CCGTTGA2750.004031151514.8403042
GCTAATT9750.014.65004351
GTCTCTA9900.014.4280731
CGGTGCG3801.4479303E-414.31959153
GCGTCAA2850.00511048314.31959151
CGGAGGT8653.6379788E-1214.1540477
Produced by FastQC (version 0.11.5)