FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896327
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG509.1079075E-487.65408145
TATACGG350.004339768659.138313
TGATCAA7950.029.5071669
GACTTGT7750.029.3783913
GAGACTT9200.029.2477531
ACTTGTG8650.027.9169314
GTGATCA8250.027.597888
CTACTCG3551.0913936E-1125.2656652
CTTGTGA9700.024.8949955
AGACTTG10350.023.9981582
GTCGAGA1450.001636736923.7912751
TTGTGAT11000.021.952866
AAAGTGC14100.021.530264
TGTGATC11700.021.229147
CGAGTAG3256.738264E-821.2291373
AAGTGCT14300.020.2641775
TACTCGG4802.7284841E-1120.1234553
GCTACTT7800.018.5754951
GCTACTC8400.018.070041
CTACTTG6500.018.0447672
Produced by FastQC (version 0.11.5)