FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896327
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG901.1532957E-681.45078145
ACGGACG951.3761382E-436.317742
CGGACGT1000.007731997427.6014823
CGACATT1050.00980902226.2871282
AAGTGCT15900.024.3031925
AAAGTGC15400.024.1961064
CCAAAGT16850.022.1139492
GCCGGAT2203.2645206E-521.9557271
GACGTTT1600.002898035321.563661
TAGCTAG1600.002898035321.563667
CTAGTCA2956.2322397E-721.051982
TAGTCAG3902.1513188E-819.4625853
CCCAAAG20300.019.0355071
CAAAGTG19400.018.851533
TATCTAG1850.00671418518.649657
ACCAACG2300.001109563518.0009699
CGAGTAG3152.5749212E-517.5247523
GGTAAAC4005.9813465E-717.2509271
CTATATA3202.9478135E-517.2509272
CCTATTA2400.001475902917.2509271
Produced by FastQC (version 0.11.5)