FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376289
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9300.030.5666642
TACTCGG10850.027.4780483
CGTATAA1901.0123111E-525.5441591
GCTACTC18650.024.5364821
CCTAACG2102.1975742E-523.111381
CGGAGGT10000.022.8802687
ACTCGGG13250.022.5008934
TCGGGAG15300.022.2050516
GCTACTT13400.021.7315961
GGACGTT1600.002819563321.666928
GGAGGCT49450.020.3305389
CCAAAGT28650.020.3283372
TTGGGAG37350.020.234066
CTACTTG13050.019.6579572
AAAGTGC25050.019.6515924
CTCGGGA15900.019.1868085
GACTTGT9600.018.7779983
GCCATAA5751.8189894E-1118.0871681
GGGAGGC66350.017.9735838
AAGTGCT27550.017.8683265
Produced by FastQC (version 0.11.5)