FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443300
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG800.002667426434.311055
CGCCGTA850.00359011432.292758
CGGAGGT17050.032.1980557
CTACTCG10450.032.176872
TACTCGG12400.028.2236063
CGCGAGT1000.00794565527.448846
AGGCGGA25200.025.0525134
ACTCGGG14200.024.6459664
GCTACTC24650.024.497951
GGAGGTT31800.023.521418
GGCGGAG27800.023.4499975
TTGGGAG43050.023.4319366
GACTTGT12700.023.2342553
ACTTGTG14700.022.8740334
TCGGGAG15900.022.8740316
GGAGGCT58650.022.8155339
CTCGGGA15950.022.3720955
GCACTTT30550.022.2376041
GGAGGCG26350.022.136162
GCGGAGG23650.022.0519226
Produced by FastQC (version 0.11.5)