FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018980
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG851.2105684E-648.9633941
GTGCGTA900.004504730530.8288084
CGGAGGT8450.029.5518747
GCGTATG950.005867790429.2062361
CGAGTAG4850.028.6040483
TGCGTAG1256.674807E-427.7459225
CTACTCG7800.025.7894822
GCCGATT1350.001046247125.6906721
TTGGGAG27650.025.0867316
CCGAGTA5950.024.4816992
TACTCGG8350.024.0907733
GGAGGCT36400.024.0108959
GCTACTC16900.023.8056761
CAACACG2800.001718283723.591213145
GCTGGGA41450.023.4284069
GTCGAGA1802.051526E-423.1216052
AGGCGGA12850.022.6717664
CCCGAGT7450.022.345711
AAAGTGC22650.021.4372484
AAGTGCT23800.021.2757635
Produced by FastQC (version 0.11.5)