FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163686
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA606.2796933E-446.0471655
TATAACG606.2796933E-446.0471654
GCGTAGT900.004599597330.698112
CTACTTG8650.030.3432182
ACGCGAG1506.120705E-527.6283026
GCTATCG1050.009763882526.3126661
GCTACTC13050.024.3467771
GCTACTT10250.024.2589951
CTACTCG5200.023.9091072
ATAGGGG3751.8798778E-423.418898145
CATAGCG3450.003185820821.212769145
GACTTGT7150.019.320493
CTCTAAT4556.2645995E-918.2164612
CTACTCA10700.018.0745892
TACTCGG6900.018.0184573
CGCTAAC400.00873313417.82040295-99
GCTAATT10550.017.6768741
CCTAAAG6355.456968E-1217.4036541
AAGTGCT21050.017.3907365
AAAGTGC20000.017.2676874
Produced by FastQC (version 0.11.5)