FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818700
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACC1055.8788704E-541.956776140-144
GTCGAGA1951.1208613E-831.8789621
GACTTGT7300.031.2239173
TGATCAA7700.029.6018949
GCTAGTA950.00599004329.0825631
CGTCGAG1205.3882884E-428.779622
CTTGTGA8700.027.7872185
TTGTGAT8800.025.9016576
AGACTTG8850.025.7553222
ACTTGTG9200.025.5262724
GAGACTT9050.025.1861441
GTGATCA9500.024.7201798
TAGCTAG2051.8771054E-523.585257
AATCGCA1550.002396433422.2809966
GGTATAG3951.0786607E-920.9836241
CTATGTA2355.3864445E-520.5743682
CTACTCG3401.0722397E-720.3150252
AAAGTGC13200.019.8841024
CCTATTA2809.255316E-619.7345961
TGTGATC12100.019.4084057
Produced by FastQC (version 0.11.5)