FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131019
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG953.4220278E-452.075253145
CTACTCG5850.036.341792
TACTCGG7250.033.233943
CTACTAG3250.032.707612
ACAATCG1750.00687885528.269424145
TCGGGAG10250.026.9638356
ACTCGGG10250.025.5810744
GCTACTC15000.025.5119361
CGGAGGT9450.025.4969377
CTCGGGA10800.024.9345075
ACGCCGG1450.001400734424.436726
GGAGGCT39100.023.9242369
CTACTTG9800.023.140082
CGAGTAG4651.2732926E-1121.3361473
GCTACTT11450.020.4244021
GCTACTA4552.0736479E-1020.2475681
TTGGGAG28800.020.1772636
CCAAAGT25150.019.4424972
GCTGGGA43750.019.4376669
CCCAAAG30500.019.2849791
Produced by FastQC (version 0.11.5)