FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065113
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGCG451.4657344E-678.246191
CGATAAA1902.2193893E-433.478924145
CGGGTTA850.00316220933.1395657
CTACTCG5650.032.406392
TACTCGG6800.028.997123
ATTCTCG2550.001241661324.945082145
CGCCCGT2750.001927219923.130894145
CCGTAAT1902.6705238E-422.2383921
ACTCGGG9550.022.1219624
CGATTAC650.00969075321.6681772
GCTACTC14450.021.4432491
AGGCGGA12300.021.1837254
CGAGTAG3801.2641976E-820.3851933
AAAGTGC18350.019.955984
CTCGGGA11350.019.854545
TTGGGAG22900.019.6811396
CTCGCGG1800.00509658819.5615482
CTACTTG9100.019.3465862
CTCCGTT2558.700703E-519.3314139
TCGGGAG11400.019.1497276
Produced by FastQC (version 0.11.5)