FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065113
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCG650.00443665558.806915145
CGGAGGT6750.035.4747777
CTAGCGC800.00234810535.2139322
CTACTCG5500.033.2931752
GCTAGCG1102.8881984E-432.012671
TACTCGG6100.028.863883
CCGCATA1800.006825119728.314442145
TATTCGG1000.006998805328.1711483
CGAGTAG5000.025.3540333
ACTCGGG7450.022.6881714
GGAGGTT18900.021.240158
GCTACTC14150.020.9043851
GTCTACG2054.4594065E-420.6130338
TCGGGAG10450.020.2185276
CCGAGTA6650.020.1222482
AGGCGGA14550.019.3616144
AGTAGCT16750.019.3413855
CCCGAGT8450.019.1697141
GCGGAGG12300.018.895286
GGAGGCG15450.018.6895962
Produced by FastQC (version 0.11.5)