FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154061
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGCG1158.246973E-443.56585145
CGTATGA2003.286511E-431.312954145
CAACGTA2155.020455E-429.128332145
CTACTCG5450.028.5539822
GCGTAGT1000.006851444528.2943972
TACTCGG6750.025.1505763
CCCGTAA1751.484415E-424.2523421
CGGAGGT8300.023.8627457
GCCGCTA1600.00251000722.1049981
CCAAAGT24550.021.6097742
CGCGGAA1650.00300081321.435155
GCTACTC14550.021.3909551
AAAGTGC20250.020.60954
CCCAAAG30650.019.847621
AGTGCGA3350.00662025718.694302145
CAAAGTG25400.018.6587073
TTGGGAG27750.018.6080286
GGAGGCT39000.018.5001839
AAGTGCT23100.018.3729845
TCTCGGA4109.823106E-418.329535145
Produced by FastQC (version 0.11.5)