FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936803
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT301.8036193E-594.355296
CGTAACG353.8754777E-580.875963
GACGCGA658.2649314E-443.5485954
CTACTCG4550.041.993292
ACGCGAG750.001670621637.7421155
GTCGAGT1852.1063216E-433.777557145
TACTCGG5750.033.2294733
CGCCATA1255.93754E-428.3065851
TGACGCG1050.00867414826.9586543
CTACTTG7700.026.6523062
GCTACTT8650.024.5432831
CCCGTAA1450.001412126124.402231
GCTACTC11500.023.9990631
ACTCGGG7550.023.4326044
CTTGTGA8800.023.3207685
CGGAGGT7050.023.0869317
GTCGAGA1550.002081695722.827892
GACTTGT7900.022.3944533
ACTTGTG9200.022.306824
TTGGGAG23750.021.7513776
Produced by FastQC (version 0.11.5)