FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936803
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG5250.032.359522
TACTCGG6500.030.4926283
CGGGTTA1255.92774E-428.314587
GTACGGG1359.293772E-426.2172034
ACTCGGG8000.025.660094
CGGAGGT7650.023.1328287
AGTAGCT12900.023.0467535
AAAGTGC17400.022.3750294
GCTACTC11500.022.1592391
CTCGGGA10000.021.94385
TACGGGT1650.002988010421.4504415
AGGCGGA12700.021.1801994
TTGGGAG22300.020.9502536
TGTACGG1700.003552669420.8195463
CCAAAGT19800.020.377922
TCGGGAG10100.020.3248256
GCTACTT10500.020.22471
CAAGTAG7450.019.9532283
AAGTGCT19600.019.8635465
GTAGCTG16050.019.8466686
Produced by FastQC (version 0.11.5)