FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398679
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1202.3875327E-741.279992
TAACCCG1204.6772513E-429.4857086
ACGTCGA1805.675931E-627.5199931
CTACTCG7800.025.4030722
CGTATAT1500.001720788723.5885681
TACTCGG9100.022.5517083
CTACTTG14050.021.1541612
TTGGGAG38600.020.7163856
GACTTGT11750.020.4768853
CTACTAG3551.3108911E-719.9340022
GCTACTT16550.019.6690181
CCTAATA5455.456968E-1219.4767991
CGAGTAG6550.019.4470633
AACCGGT1850.00580606919.1258668
ACTTGTG13850.018.9049174
GGAGGCT52950.018.710489
CCAAAGT30300.018.6840132
CTACGTG3051.571512E-518.5614952
CCTTACG2309.371375E-418.4606191
GAGACTT13500.018.3466641
Produced by FastQC (version 0.11.5)