FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953531
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG1001.823248E-434.621118
ACGACAG2501.9222578E-532.731644145
AGCGTAA1050.00393672731.688812135-139
TTGTGCG1454.775941E-528.6519494
CTACTCG5400.026.9275262
GCTACTT8950.024.7569921
TAGGGCG1400.001306723724.729365
GCGAAAA1450.001603047523.8766251
TACTCGG7000.021.7618393
CGGAGGT7050.021.60757
GCTACTC15050.021.1637331
CTACTTG8150.020.3903452
TTGGGAG25250.020.0185416
GATAATT4552.8376235E-1019.7834911
CCTAATA2508.50698E-519.3878211
AAGATCG4257.053387E-419.253906145
GCTGGGA34950.019.0193219
TTAATCA4358.2476094E-418.811289145
AGTAGCT14600.018.4962085
AAAGTGC16700.018.2434564
Produced by FastQC (version 0.11.5)