FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953531
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG6200.033.5431562
GCGGTAA900.00451804630.810011
TTGGGAG25650.027.5668496
TACTCGG7850.027.3757723
GTCGGTT1308.4234733E-426.662511
GCGGGTA2050.00917971626.644707145
GCACTTT15350.026.1935231
TCGGGAG9650.025.861256
GGAGGCT31550.024.8286699
CTCGGGA10750.024.5047055
GCTACTC13550.023.5338441
ACTCGGG10150.023.2213384
TTTGGGA22150.022.84675
CGAGTAG4950.022.407283
CTACTTG8900.021.8093322
GGGAGGC44050.021.0878948
GCTACTT9350.020.7596841
ACTTTGG19550.020.5662753
CACTTTG20250.020.1976742
CGGAGGT7650.019.9358887
Produced by FastQC (version 0.11.5)