FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446841
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9900.034.8286932
TACTCGG11050.033.1144373
GCTACTC22100.025.1542341
ACTCGGG14700.023.9347524
CTACTTG16350.023.2408642
CGAGTAG5600.022.6183413
GCCGGTA1600.002587465821.9900539
CGGAGGT12300.021.7397617
GCTACTT17850.021.6820721
TCGGGAG16650.021.5542166
CTCGGGA17750.020.2184625
TTGGGAG39150.019.7713896
GGAGGCT55850.019.5292179
AGGCGGA19250.019.3740944
AAAGTGC25300.019.1913194
AGTAGCT23900.018.5489335
GGAGGTT29300.018.2524958
GCTGGGA53150.018.005789
GCACTTT25700.017.7973981
CGGTGTA2000.00940323117.5920453
Produced by FastQC (version 0.11.5)