FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371857
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1352.6320626E-531.2671132
GCGTATG1307.7309005E-427.0580771
CTACTCG8600.022.0869432
CGAGTAG6400.021.9846883
AAAGTGC24450.021.8677984
AAGTGCT23750.021.6236785
CGTATGG1650.003097620621.3184872
AGTAGCT22500.021.2616375
TTGGGAG33700.021.0844276
GTCGAGA2705.770753E-620.8447421
GCTACTC19600.020.1002861
AGTGCGA3200.004581278719.924486145
CTACTAG3855.704346E-419.872734145
CCGCTAT2156.19411E-419.632841
GCCGATA1800.005126956419.5419461
CGGAGGT11950.019.4274757
GCTGGGA50800.019.3880739
GACTTGT12400.019.2897913
CCCGAAT2601.0180019E-418.9406551
TACTCGG10150.018.714063
Produced by FastQC (version 0.11.5)