FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753540
Sequences flagged as poor quality0
Sequence length35-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG204.5552003E-4104.353773
ATGCGCG350.004199333559.6307262
TAACGGG900.00443963230.9196345
GCCGATT900.00443963230.9196349
GTAACGG900.00443963230.9196344
CCCGTAA1000.007429351627.8276711
GACTTGT4550.025.992883
TCGTTGT1550.00229829422.441673
AATGCCG1550.00229829422.441676
CCGGGTT3755.14774E-1022.2621368
ACTTGTG6300.022.0854534
CTACTTG6600.021.081572
GCGCACT1650.003303639121.081575
CTACTCG5350.020.8057372
TACTCGG5700.020.7487013
CGGAGGT6450.020.4932467
ATCGGCC1700.00392753220.4615253
CTTGTGA6800.019.4384485
TTGGGAG21150.018.749146
TGATCAA5951.8189894E-1218.7076789
Produced by FastQC (version 0.11.5)