FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207703
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTA1851.6303663E-435.283897145
CTACTCG7650.032.007282
CGTCGAG1205.0016626E-429.1494852
TACTCGG9150.027.524763
GCTACTC17450.025.6582281
ACTCGGG11250.023.008664
TCGGGAG12850.022.3214726
GCCGATA1650.003199486821.1996251
AGTAGCT18100.020.8716745
CTCGGGA13700.020.4259155
CGAGTAG4852.7284841E-1120.1942833
AAAGTGC22450.019.9436994
TTGGGAG32200.019.7709546
GCTGGGA43900.019.7605639
GGAGGCT44950.018.9876969
GCTACTT12950.018.9077741
CCAAAGT27450.018.6046982
GCTATAT4957.6397555E-1018.3730091
GCACTTT21400.018.3069651
AAGTGCT24050.018.0351095
Produced by FastQC (version 0.11.5)