FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205709
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8800.032.6113472
CTACGTA900.00431002531.1089291
CCCGTAA1653.1396048E-629.6948831
TACTCGG10350.029.0800843
GCTACTC18850.028.9634861
AGTAGCT17900.023.8530755
TACGGGT1500.001833830823.3316965
CGTAAAG1852.3233691E-422.7011073
ACTCGGG12050.022.654014
CCGTAAA1550.002218983622.579062
CGGAGGT10550.022.557667
TTGTCGC1902.782837E-422.1037126
CTACTCA11200.021.8734632
TTGGGAG31650.021.2307366
TCGGGAG13900.021.1495956
GTAGCTG20550.020.777136
CTCGGGA14250.020.630135
TCGCGGG1700.00379241220.586795
GGTATAT4903.092282E-1119.9985961
AAAGTGC22800.019.954744
Produced by FastQC (version 0.11.5)