FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900511
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT5700.023.3812527
CGAGTAG4355.456968E-1222.5753
CTACTCG5001.8189894E-1221.0431252
GCCGGCG2405.6263823E-520.4585951
GGAGGCT31100.019.847759
AGTAGCT17000.019.8052965
TTGGGAG23200.019.6523466
GCACTTT15300.019.2551481
GTAGCTG18300.019.164966
GCTACTC11600.018.7453141
TACTCGG6001.8189894E-1218.7053
GGAGGTT15700.018.3177958
CCGAGTA5451.364242E-1018.0185782
AAAGTGC16500.017.8547744
TAGCTGG20050.017.4922087
ACTTTGG18650.017.3008733
GCTACTT9150.016.8651661
AGGCGGA10000.016.83454
GCTGGGA37350.016.7143089
GGTAATA4701.5061232E-716.4166241
Produced by FastQC (version 0.11.5)