FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734148
Sequences flagged as poor quality0
Sequence length35-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4150.030.389232
CGAGTAG3104.3655746E-1127.121573
GGCGGTA1307.914182E-426.9477141
GCTACTC8950.026.6165121
TACTCGG5400.025.949653
CGGAGGT6150.025.0635647
GAGTAGC6850.024.547994
ACTCGGG6500.023.7139914
CCGAGTA3953.6379788E-1123.0590572
AGGCGGA9950.021.8290044
CTCGGGA8450.021.5581725
AGTAGCT10400.021.5581725
TCGGGAG7500.021.4863116
GCTGGGA26650.021.2952679
CTACTTG5600.021.2694472
AAAGTGC13600.021.1222524
GCTACTT6750.020.759721
GGAGGCG10150.020.7085882
AAGTGCT14400.020.435355
GGAGGCT25450.020.3722619
Produced by FastQC (version 0.11.5)